Comparative Genomic Hybridization Analysis of Adrenocortical Tumors

Comparative genomic hybridization analysis of adrenocortical tumors of childhood.

Although several genes have been investigated in adrenal tumorigenesis, the genetic background of adrenocortical tumors (ACT) remains poorly characterized. In southern Brazil, the annual incidence of ACT is unusually high, ranging from 3.4-4.2/million children, compared with a worldwide incidence of 0.3/million children younger than 15 yr. Environmental factors have been implicated because the ...

Comparative genomic hybridization for molecular cytogenetic analysis of solid tumors.

Comparative genomic hybridization produces a map of DNA sequence copy number as a function of chromosomal location throughout the entire genome. Differentially labeled test DNA and normal reference DNA are hybridized simultaneously to normal chromosome spreads. The hybridization is detected with two different fluorochromes. Regions of gain or loss of DNA sequences, such as deletions, duplicatio...

Genetic aberrations in adrenocortical tumors detected using comparative genomic hybridization correlate with tumor size and malignancy.

The differentiation between malignant and benign adrenocortical tumors is often difficult, and better markers are required. Because the genetic background of adrenocortical tumors is poorly characterized, we used comparative genomic hybridization (CGH) to screen for DNA sequence copy number changes in 8 sporadic primary adrenocortical cancers and 14 adenomas. There was a strong relationship bet...

Genomic analysis of tumors by array comparative genomic hybridization: more is better.

We read with interest the article by Baldwin et al. (1), which describes the use of an array of 32,433 bacterial artificial chromosome clones tiled across the genome (2, 3) to map genomic aberrations in 20 oral squamous cell carcinoma samples by array comparative genomic hybridization (array CGH). The authors conclude that improved detection sensitivity and resolution of tiling arrays is necess...

Comparative Genomic Hybridization (CGH)